Isolation and Chromosomal Localization of Highly Repeated DNA Sequences in Drosophila melanogaster
- 1 June 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (6) , 1125-1129
- https://doi.org/10.1073/pnas.68.6.1125
Abstract
The nuclear DNA of D. melanogaster contains DNA sequences that are repeated between ten and a hundred times more often than the next class of redundant DNA. This DNA, as a renatured duplex isolated on the basis of its renaturation kinetics, has a buoyant density of 1.691 (g/ml). In its native state it bands within the unique nuclear DNA peak (rho = 1.701). These sequences have been localized by "in situ" hybridization in the chromocenter of the chromosomes of the salivary gland. The properties of centromeres are discussed in terms of the occurrence of repeated sequences at this locus.Keywords
This publication has 16 references indexed in Scilit:
- Chromosomal Localization of Mouse Satellite DNAScience, 1970
- Chromosomal and Nuclear Location of Mouse Satellite DNA in Individual CellsNature, 1970
- Variations in the DNA from Two Rodent Families (Cricetidae and Muridae)Nature, 1970
- A New Method for the Large Scale Purification of Escherichia coli Deoxyribonucleic Acid-dependent Ribonucleic Acid PolymeraseJournal of Biological Chemistry, 1969
- Renaturation and isolation of single strands from the nuclear DNA of the guinea pigJournal of Molecular Biology, 1969
- Some properties of the single strands isolated from the DNA of the nuclear satellite of the mouse (Mus musculus)Journal of Molecular Biology, 1969
- Isolated mammalian metaphase chromosomes: II. Fractionated chromosomes of mouse and Chinese hamster cellsJournal of Molecular Biology, 1969
- Repeated Sequences in DNAScience, 1968
- Kinetics of renaturation of DNAJournal of Molecular Biology, 1968
- Nucleotide Sequence Repetition: A Rapidly Reassociating Fraction of Mouse DNAScience, 1966