Expression of Cre recombinase in the developing mouse limb bud driven by a Prxl enhancer
Top Cited Papers
- 8 May 2002
- Vol. 33 (2) , 77-80
- https://doi.org/10.1002/gene.10092
Abstract
Summary: We have used a Prx1 limb enhancer to drive expression of Cre Recombinase in transgenic mice. This regulatory element leads to Cre expression throughout the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. Crossing a murine line carrying this transgene to a reporter mouse harboring a floxed Cre‐reporter cassette revealed that recombinase activity is first observed in the earliest limb bud at 9.5 dpc. By early to mid bud stages at 10.5 dpc recombination is essentially complete in all mesenchymal cells in the limb. Expression of the Cre recombinase was never detected in the limb bud ectoderm. The use of Prx1–Cre mice should facilitate analysis of gene function in the developing limb. genesis 33:77–80, 2002.Keywords
This publication has 13 references indexed in Scilit:
- FGFR3 targeting strategies for achondroplasiaExpert Reviews in Molecular Medicine, 2012
- Patterning Mechanisms Controlling Vertebrate Limb DevelopmentAnnual Review of Cell and Developmental Biology, 2001
- Cholesterol Modification of Sonic Hedgehog Is Required for Long-Range Signaling Activity and Effective Modulation of Signaling by Ptc1Cell, 2001
- Cre recombinase: The universal reagent for genome tailoringGenesis, 2000
- Z/AP, a Double Reporter for Cre-Mediated RecombinationDevelopmental Biology, 1999
- Paired-Related Homeobox Genes Cooperate in Handplate and Hindlimb Zeugopod MorphogenesisDevelopmental Biology, 1999
- The paired-like homeo box gene MHox is required for early events of skeletogenesis in multiple lineages.Genes & Development, 1995
- The Expression Pattern of the Chick Homeobox Gene gMHox Suggests a Role in Patterning of the Limbs and Face and in Compartmentalization of SomitesDevelopmental Biology, 1994
- A 5′ element of the chicken β-globin domain serves as an insulator in human erythroid cells and protects against position effect in DrosophilaCell, 1993
- A Chicken Homeobox Gene Related to Drosophila paired Is Predominantly Expressed in the Developing LimbDevelopmental Biology, 1993