Changes in synthesis of types-I and -III collagen during matrix-induced endochondral bone differentiation in rat

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Abstract
The changes in rates of hydroxyproline formation and biosynthesis of types I and III collagen during bone-matrix-induced sequential differentiation of cartilage, bone and bone marrow in rat were investigated. Biosynthesis of types I and III collagen at different stages of this sequence was studied by labeling in vivo and in vitro with [2,3-3H]proline. Pepsin-solubilized collagens were separated by sodium dodecyl sulfate/polyacrylamide-slab-gel electrophoresis. Maximal amounts of type III collagen were synthesized on day 3 during mesenchymal cell proliferation. Thereafter, there was a gradual decline in type III collagen synthesis. On days 9-20 during bone formation predominantly type I collagen was synthesized. Similar results were obtained by the use of labeling techniques in vivo and in vitro.