Studies on the Regeneration of Apple Cultivars from Root Cuttings.

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Abstract
Summary A technique was developed for producing established apple trees on their own roots. They were derived from adventitious shoots regenerating from scion cultivar root cuttings. Provided they were excavated in late autumn, root cuttings of such cvs as Lord Lambourne and Lord Derby (15 cm long and 1.5 cm diam.) could produce up to ten shoots each and survive for up to nine months. Regeneration vigour could be increased with cold storage, and treatment of cuttings with cytokinin. When young shoots (3–6 cm long) were detached and treated with 50 mg 1-1 IBA, approximately 90 % rooting was achieved under mist, although the final establishment rate was somewhat lower. Anatomically, adventitious buds arose spontaneously and at random in the cortex parenchyma and only later became continuous with the vascular cambium. For commercial use the practical and economic aspects of this technique require further investigation.