Conservation of structure and function of DNA replication protein A in the trypanosomatid Crithidia fasciculata.
- 1 November 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (21) , 10227-10231
- https://doi.org/10.1073/pnas.89.21.10227
Abstract
Human replication protein A (RP-A) is a three-subunit protein that is required for simian virus 40 (SV40) replication in vitro. The trypanosome homologue of RP-A has been purified from Crithidia fasciculata. It is a 1:1:1 complex of three polypeptides of 51, 28, and 14 kDa, binds single-stranded DNA via the large subunit, and is localized within the nucleus. C. fasciculata RP-A substitutes for human RP-A in the large tumor antigen-dependent unwinding of the SV40 origin of replication and stimulates both DNA synthesis and DNA priming by human DNA polymerase alpha/primase, but it does not support efficient SV40 DNA replication in vitro. This extraordinary conservation of structure and function between human and trypanosome RP-A suggests that the mechanism of DNA replication, at both the initiation and the elongation level, is conserved in organisms that diverged from the main eukaryotic lineage very early in evolution.Keywords
This publication has 30 references indexed in Scilit:
- Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase.Genes & Development, 1991
- Class I and class II ribonuclease H activities inCrithidia fasciculata (protozoa)Cellular and Molecular Life Sciences, 1991
- Cell Cycle Control of DNA Replication by a Homologue from Human Cells of the p34
cdc2
Protein KinaseScience, 1990
- Cell-cycle-regulated phosphorylation of DNA replication factor A from human and yeast cells.Genes & Development, 1990
- Binding and unwinding—How T antigen engages the SV40 origin of DNA replicationCell, 1990
- Replication of Kinetoplast DNA in TrypanosomesAnnual Review of Microbiology, 1988
- An Origin Unwinding Activity Regulates Initiation of DNA Replication During Mammalian Cell CycleScience, 1988
- Purification and nuclear localization of a type I topoisomerase from Crithidia fasciculataMolecular and Biochemical Parasitology, 1987
- Isolation and partial characterization of DNA polymerases from Crithidia fasciculataMolecular and Biochemical Parasitology, 1984
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970