Curing effect of clorobiocin on Escherichia coli plasmids
- 1 June 1982
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 186 (1) , 153-155
- https://doi.org/10.1007/bf00422928
Abstract
Clorobiocin, an inhibitor of the gyrB subunit of DNA gyrase, was used for the curing of some Escherichia coli plasmids. Of the plasmids studied, ampicillin resistant R28K and a miniplasmid derived from R1 drd-19 were effectively eliminated. We also succeeded in eliminating the ColA factor from E. coli strain B834 (pBS103), which was resistant to the effect of currently used curing agents. Although a derivative of ColE1-pBR322 was effectively cured by clorobiocin, the ColE1-plasmid was resistant to its effect. The ColV plasmid determining virulence was effectively eliminated.This publication has 7 references indexed in Scilit:
- Curing of Escherichia coli K12 plasmids by coumermycinMolecular Genetics and Genomics, 1980
- [Specification of DNA-cytosine-methylase I (M.Eco MRE 600 I) by plasmid Col A].1980
- Characterization of a plasmid mutation affecting maintenance, transfer and elimination by novobiocinMolecular Genetics and Genomics, 1979
- A rapid method for the identification of plasmid desoxyribonucleic acid in bacteriaPlasmid, 1978
- Elimination of Plasmids from Several Bacterial Species by NovobiocinAntimicrobial Agents and Chemotherapy, 1977
- The elimination of extrachromosomal elements in thymineless strains of Escherichia coli K12Genetics Research, 1965