Inhibition of dolichyl phosphate biosynthesis by compactin in cultured rat hepatocytes

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Abstract
Isolated rat hepatocytes were cultured in monolayer for about 24 h. During this period the cells exhibited constant protein and lipid synthesis. When the culture medium contained compactin, a competitive inhibitor of the 3‐hydroxyl‐3‐methylglutary‐coenzyme‐A reductase, dolichyl‐P synthesis was inhibited by 91% at the end of the incubation, as estimated by the incorporation of [3H]acetate and by 77% as estimated by the incorporation of 32Pi. These results indicate that in primary cultures of rat hepatocytes dolichyl monophosphate is mainly synthesized through a de novo process, while phosphorylation through the CTP‐mediated kinase is of limited functional importance.