Estimation of Airborne Rat-Derived Antigens by ELISA

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Abstract
Double antibody sandwich ELISA procedures have been devised to estimate the concentrations of airborne rat urinary and epidermal antigens. Both assays were capable of detecting at least 3 ng/ml of antigen protein. Unknown concentrations were estimated in terms of the standard, laboratory-prepared material, as calculated from the linear relationship between absorbance and log concentration. The coefficient of variation of a single assay was 12%, the methods have been applied to extracts of the filter material from static and personal samplers in an animal room.