ENUMERATION OF HEMATOPOIETIC PROGENITORS (CFU-C AND BFU-E) IN LIQUID MICROCULTURE USING A LIMITING DILUTION ANALYSIS

  • 1 January 1980
    • journal article
    • research article
    • Vol. D131  (1) , 111-115
Abstract
To overcome the difficulty of scoring multicentric colonies of [mouse] hemopoietic progenitor cells, cultures in 10 .mu.l of liquid medium were grown. Plated at 3 different cell concentrations, progenitor cells (CFU-C [granulocytic progenitor cells] or BFU-E [erythropoietic burst-forming units]) are not present in all wells. Their presence is easily scored in each well of microculture 7 days after incubation. A limiting dilution analysis allows accurate quantification.

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