Preparation of Methyl Derivatives of Some Organic Acids for Analysis by Gas–Liquid Chromatography
- 1 August 1971
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 49 (8) , 949-952
- https://doi.org/10.1139/o71-137
Abstract
One microgram to 1 mg of organic acid was completely converted to its methyl derivative when it was dissolved in methanol and treated with an excess of ethereal diazomethane. Single products were obtained from α-ketoglutaric acid, oxaloacetic acid, pyruvic acid, glyoxylic acid, or hydroxypyruvic acid on reaction with thionyl chloride – methanol. The separation of 24 organic acids by gas–liquid chromatography on a Reoplex 400 column is presented.Keywords
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