Dube, Volker E., House, Roy F., Moulds, John, and Polesky, Herbert F.: Hemolytic anemia caused by auto anti-N. Am J Clin Pathol 63: 828–831, 1975. The second case of hemolytic anemia caused by auto anti-N, occurring in a 7-year-old boy, is described. The antibody was IgG, as shown by the use of specific anti-human IgG Coombs sera, failure of inactivation by 2-mercaptoethanol, and chromatographic separation on a G-200 Sephadex column.