Antibodies to Histones and Histone-Histone Complexes: Immunochemical Evidence for Secondary Structure in Histone 1
- 30 April 1976
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 192 (4238) , 469-471
- https://doi.org/10.1126/science.3853
Abstract
Highly specific antibodies were raised to histone 1 (H 1) and the histone complexes H32-H42 AND H2A-H2B, isolated by salt extraction. Antibody to H1 could detect irreversible conformational changes in acid- or urea-treated H1. The antibodies showed different reactivities with chromosomes as compared to antibodies in acid-extracted histones and should be useful in studies of native chromatin and chromosome structure.Keywords
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