Replication of plasmids with the p15A origin in Shewanella putrefaciens MR‐1

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Abstract
The plasmid pACYC184 was introduced into Shewanella putrefaciens MR‐1 by electroporation. In 100% of the transformants examined, the plasmid was maintained as a free replicon outside the chromosome. This was the case whether or not the plasmid contained a 224‐bp DNA insert derived from an open‐reading frame of MR‐1 genomic DNA. Therefore, in contrast to a report in the literature, plasmids containing the p15A origin of replication can replicate freely in S. putrefaciens MR‐1, and do not make convenient vectors for gene replacement in this bacterium. However, we found that plasmids with the pMB1 origin of replication (e.g. pBR322) cannot replicate in MR‐1 and could therefore have potential as vectors for gene replacement.

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