INDUCTION OF GAMMA-GLUTAMYL-TRANSFERASE TRANSPEPTIDASE POSITIVE FOCI IN RAT-LIVER BY PYROLYSIS PRODUCTS OF AMINO-ACIDS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 72  (5) , 747-753
Abstract
The activities of pyrolysis products for initiating or promoting preneoplastic lesions were tested. In experimental series I, N-2-fluorenylacetamide (2-FAA) was used as an initiator or promoter and 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), 2-amino-6-methyldipyrido[1,2-a:3'',2''-d]imidazole (Glu-P-1) or 2-amino-dipyrido[1,2-a:3'',2''-d]imidazole (Glu-P-2) was administered at a fixed dose and for the same period in the initiation stage or the promotion stage. Animals were subjected to partial hepatectomy or treated with CCl4 to increase the induction of .gamma.-glutamyl transpeptidase (.gamma.-GT) positive foci. Trp-P-1 and Glu-P-2 administered in the initiation or promotion stage significantly increased the induction of .gamma.-GT positive foci (P < 0.001). Glu-P-1 increased the induction of .gamma.-GT positive foci (P < 0.001) when administered in the initiation stage but was not effective when administered during the promotion stage. In experimental series II, animals were given a single dose of these chemicals at 1 of 3 different dose levels with partial hepatectomy and were fed with 2-FAA and given a single dose of CCl4. Trp-P-1, Glu-P-1 and Glu-P-2 caused dose-dependent inductions of .gamma.-GT positive foci.