Method Comparison for Total Plasma Homocysteine between the Abbott IMx Analyzer and an HPLC Assay with Internal Standardization

Abstract
The increasing interest in measuring total homocysteine in plasma has led to the development of several different methods (1). Although most publications describe HPLC methods with manual sample preparation, the first fully or partially automated kits were introduced recently: a fluorescence polarization immunoassay on the IMx® analyzer (Abbott Laboratories, Abbott Park, IL), a microtiter plate enzyme immunoassay (Bio-Rad Laboratories), and an HPLC kit with electrochemical detection (BAS). We evaluated the Abbott IMx analyzer automated method for total homocysteine and compared it with our HPLC assay with internal standardization.