A Mixture of Methyl Green and Pyronin for Cell Fractionation Studies
- 1 January 1953
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 28 (1) , 41-44
- https://doi.org/10.3109/10520295309112634
Abstract
A staining mixture consisting of 0.57% methyl green and 0.1 1 % pyronin B (calculated from the actual dye content) dis- solved in glycerol, 20 ml.; 2% aqueous phenol, 100 ml.; and 95% ethanol, 25 ml., was found to be optimum for differentiating cell components containing desoxypentose and pentose nucleic acids. The stain can be used for either fresh suspensions or unfixed dried smears of tissue homogenates. Nuclei are stained bright blue, and nucleoli and cytoplasmic particles, bright pink.Keywords
This publication has 5 references indexed in Scilit:
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- METHYL GREEN-PYRONINThe Journal of general physiology, 1950
- The Nature of the Specificity of Methyl Green for ChromatinProceedings of the National Academy of Sciences, 1949
- ISOLATED CHROMOSOMESThe Journal of general physiology, 1947
- A Differential Stain Favorable to the Diagnosis of Neisserian InfectionStain Technology, 1931