EFFECT OF PHOSPHOLIPID ON ALDOSTERONE BIOSYNTHESIS BY A CYTOCHROME-P-45011-BETA-RECONSTITUTED SYSTEM

  • 1 January 1984
    • journal article
    • research article
    • Vol. 9  (6) , 715-723
Abstract
Effect on phospholipid on aldosterone synthesis catalyzed by a cytochrome P-45011.beta.-reconstituted system was examined. Corticosterone was incubated with P-45011.beta. in the presence of phosphatidylcholine, phosphatidylethanolamine, cardiolipin, phosphatidylinositol or phosphatidylserine. These phospholipids, stimulated the rate of aldosterone- and 18-hydroxycorticosterone-synthesis, although the mode of stimulation by neutral phospholipids was different from that by acidic phospholipids. In the presence of a phospholipid mixture containing the lipids in the same molar ratios as found in [bovine] adrenocortical mitochondria, the rate of production of aldosterone increased 10-fold over the rate without the lipids, and that of 18-hydroxycorticosterone increased 3-fold. The maximal synthetic rates of aldosterone and 18-hydroxycorticosterone were 2 and 5 nmol/nmol P-450 per min, respectively.