The Chemistry of Olfactory Reception: Stimulus-Specific Protection from Sulfhydryl Reagent Inhibition

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Abstract
The group-specific protein reagent, N-ethylmaleimide, irreversibly blocks the electrical response of the olfactory receptor organ of the frog to odorous stimuli. If the odorous substance, ethyl n-butyrate, in concentrations high enough to saturate the receptor system, is present in the nasal cavity before and during a brief exposure to N-ethylmaleimide, the nose, after a wash and a recovery period, responds in nearly normal fashion to vapors of ethyl n-butyrate. Responses to other odorous substances, except those closely related to ethyl n-butyrate, are abolished. We propose that we can use this protection technique to identify the properties of the various receptor sites in the nose, and possibly to characterize the receptor substances.