Characterization of Fat Particles in Plasma of Hyperlipemic Subjects Maintained on Fat-free High-Carbohydrate Diets*

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Abstract
In plasma of hyperlipemic subjects on ordinary diets, a mixture of fat particles can be found with propteries similar to primary and secondary particles observed in normal subjects during alimentary lipemia. However, when fat is excluded from the diet for prolonged periods, plasma from patients who maintain gross hyperlipemia contains only one type of particle distinctly different from those recovered following fat ingestion. This "hyperlipemic" particle is unusually rich in cholesterol and, although grossly visible (Sf > 400), resembles very low density lipoproteins (Sf20 to 400) more closely than other lipid-protein aggregates. Fat particle mixtures in plasma can be fractionated by a combination of methods, including zone electrophoresis on nonadsorptive media, ultracentrifugation, and polyvinylpyrrolidone flocculation.