Diffusion of the mu opioid receptor at the surface of human neuroblastoma SH‐SY5Y cells is restricted to permeable domains
- 5 September 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (22) , 5227-5231
- https://doi.org/10.1016/j.febslet.2006.08.056
Abstract
Previous single-molecule studies have shown a long-term diffusion superimposed to a short-term confinement of the human mu opioid (hMOP) receptors at the surface of heterologous cells. However, additional ensemble average measurements are required to reach a complete understanding of the undergoing process. Here, we analyse, by fluorescence recovery after photobleaching measurements, the lateral diffusion of fully functional T7-EGFP-hMOP receptors in neuroblastoma SH-SY5Y cells naturally expressing a low level of the wild-type receptor. Experiments carried out at variable observation radii demonstrate the restriction of the receptors diffusion to sub-micrometer sized domains. Furthermore, consistently with the long-term single-molecule data, the domains are found permeableKeywords
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