Repair Kinetics of Abasic Sites in Mammalian Cells Selectively Monitored by the Aldehyde Reactive Probe (ARP)
- 1 January 1998
- journal article
- research article
- Published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids
- Vol. 17 (1) , 503-513
- https://doi.org/10.1080/07328319808005194
Abstract
Human methylpurine N-glycosylase (MPG) activity was investigated by monitoring abasic (AP) sites resulting from removal of alkylated bases. The amount of AP sites in MMS-treated HeLa cells transiently increased at 3 h, then gradually decreased to 40% at 24 h. The presence of adenine, an inhibitor of AP endonucleases, in the repair incubation of MMS-treated cells induced moderate accumulation of AP sites, suggesting inhibition of the activities of MPG as well as AP endonucleases by adenine metabolites.Keywords
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