A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution
Open Access
- 1 March 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (6) , 1309-1310
- https://doi.org/10.1093/nar/25.6.1309
Abstract
A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5′-amino-5′-deoxy-thymidine is described. A 3′-phosphorylated ODN (the donor) is incubated in aqueous solution with 5′-amino-5′-deoxythymidine in the presence of N-(3-dimethyl-aminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond. Template- directed ligation of the extended donor and an acceptor ODN, followed by acid hydrolysis, yields the acceptor ODN extended by a single 5′-amino-5′-deoxythymidine residue at its 5′ terminus.Keywords
This publication has 8 references indexed in Scilit:
- Template-directed ligation of peptides to oligonucleotidesChemistry & Biology, 1996
- Preparation of oligonucleotide-peptide conjugatesBioconjugate Chemistry, 1991
- The synthesis of protected 5′-amino2′, 5′-dideoxyribonucleoside-3′-O-phosphoramidites; applications of 5′-amino-oligodeoxyribonucleotidesNucleic Acids Research, 1987
- [19] The synthesis and use of fluorescent oligonucleotides in DNA sequence analysisPublished by Elsevier ,1987
- The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysisNucleic Acids Research, 1985
- Derivatization of unprotected polynucleotidesNucleic Acids Research, 1983
- Incorporation of 5'-amino-5'-deoxythymidine 5'-phosphate in polynucleotides by use of DNA polymerase I and a φX174 DNA templateBiochemistry, 1976
- Nucleotide chemistry. XVI. Phosporamidate analogs of oligonucleotidesThe Journal of Organic Chemistry, 1970