Rapid Decalcification for Histochemistry

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Abstract
Only.[long dash]A method for the rapid and gentle decalcification of bone or teeth for the purpose of histochemicarenzyme localizations, employing an "Ionic Bone Decalcifier" apparatus in combination with a decalcifying fluid, ethylenediaminetetraacetic acid (EDTA). The method permits complete decalcification of bone specimens as large as an entire hamster skull (25 mm long axis) in less than 12 hr. below 10[degree]C. The decalcified specimens are then frozen in a liquid protein matrix at -20[degree]C and sectioned at 10 [mu] on a Lab-Tek cryostat. The chosen histochemical procedure is then performed, or the sections are fixed first in 50% glutaraldehyde. Either method allows excellent histochemical enzyme localizations, although better cellular detail is achieved by the latter.

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