Isolation and characterisation of a functional αβ heterodimer from the ATP synthase of Rhodospirillum rubrum
- 28 September 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 310 (2) , 187-192
- https://doi.org/10.1016/0014-5793(92)81326-h
Abstract
An αβ heterodimer of the F1‐ATPase of Rhodospirillum rubrum was isolated by extraction of chromatophores with LiCl. Each αβ heterodimer contains one tightly bound ADP, which is released upon removal of medium Mg2+. The dimer can be reversibly dissociated by removal of Mg2+‐ions. The αβ heterodimer restores both ATP‐synthetic and hydrolytic activities to LiCl‐treated chromatophores, saturation being achieved at approximately 2 mmol αβ · mol BChl−1. The heterodimer itself hydrolyses Mg‐ATP with an activity distinct from RF1, being unaffected by azide or sulphite ions. The V max and K m (ATP) for this Mg2+‐dependent activity were 110 ± 10 nmol · min−1 mg protein−1 and 100 ± 30 μM, respectively. The K m did not differ significantly from that of RF1.Keywords
This publication has 40 references indexed in Scilit:
- Small-angle neutron scattering from the reconstituted TF1 of H+-ATPase from thermophilic bacterium PS3 with deuterated subunitsJournal of Molecular Biology, 1990
- Photoaffinity cross‐linking of F1ATPase from the thermophilic bacterium PS3 by 3′‐arylazido‐β‐alanyl‐2‐azido ATPFEBS Letters, 1989
- α3β3 complex of thermophilic ATP synthase Catalysis without the γ‐subunitFEBS Letters, 1989
- Inorganic pyrophosphate‐driven ATP‐synthesis in liposomes containing membrane‐bound inorganic pyrophosphatase and F0‐F1 complex from Rhodospirillum rubrumFEBS Letters, 1983
- Interconversion of two kinetically distinct states of the membrane‐bound and solubilised H+‐translocating ATPase from Rhodospirillum rubrumFEBS Letters, 1977
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Purification and Properties of a Coupling Factor (Ca2+‐Dependent Adenosine Triphosphatase) from Rhodospirillum rubrumEuropean Journal of Biochemistry, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Studies on bacterial photophosphorylation IV. On the maximum amount of delayed photophosphorylation induced by a single flashBiochimica et Biophysica Acta, 1962
- Studies on bacterial photophosphorylation III. A sensitive and rapid method of determination of photophosphorylationBiochimica et Biophysica Acta, 1962