Comparison of Egg-Yolk and Serum Antibody Titers to Four Avian Viruses by Enzyme-Linked Immunosorbent Assay Using Paired Field Samples

1 October 1989

journal article
research article
Published by JSTOR in Avian Diseases

Vol. 33 (4) , 643-648
https://doi.org/10.2307/1591138

Abstract

Eggs and blood were collected from 11 hens in each of nine broiler-breeder flocks in Quebec. Serum and egg-yolk extracts were assayed for antibody titers to infectious bursal disease virus (IBDV), infectious bronchitis virus (IBV), Newcastle disease virus (NDV), and reovirus (RV) by a commercial enzyme-linked immunosorbent assay (ELISA) kit. Comparison was made between egg yolk and serum antibody titers by a regression analysis. A high correlation was observed between serum and yolk antibody titers to all the viruses tested (r = 0.9 for IBDV, 0.84 for IBV, 0.84 for NDV, and 0.91 for RV). Antibody monitoring of commercial breeder flocks using egg yolk instead of serum with commerical ELISA plates is thus feasible and is recommended.

This publication has 0 references indexed in Scilit: