Isolation and Immunochemical Properties of Haptenic Material from Timothy Pollen

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Abstract
Summary: The isolation of a fraction (WST-Fr2) from the dialysate of timothy grass pollen extract is described. This fraction (WST-Fr2) appears homogenous by chromatography on Sephadex-G25, Amberlite IRC-50 and electrophoresis, but immunologically heterogenous as demonstrated by inhibition of several lines of precipitation in agar diffusion. WST-Fr2 possesses haptenic properties as demonstrated by its inhibition of specific precipitation and inhibition of the formation of lines of precipitation in agar diffusions. The results indicate the importance of considering the possible presence and significance of haptenic material in pollen extracts for laboratory studies as well as for clinical use.

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