PLATELET-DERIVED GROWTH-FACTOR PROMOTES HUMAN PERIPHERAL MONOCYTE ACTIVATION

  • 1 January 1985
    • journal article
    • research article
    • Vol. 66  (1) , 179-183
Abstract
Like in the polymorphonuclear leukocyte (PMN), the platelet-derived growth factor (PDGF) purified to homogeneity is capable of inducing monocyte activation responses as evaluated by generation of superoxide anion .**GRAPHIC**. from membrane-associated oxidase system, release of granule enzymes and enhanced cell adherence and cell aggregation. .**GRAPHIC**. release was maximized at 10 ng/ml PDGF and was comparable to that induced by 10-7 mol/l formylmethionylleucylphenylalanine. The potency of PDGF to induce this response in monocytes was of the same magnitude as that observed in PMN. Lysozyme release and monocyte adherence were also increased in a dose-dependent manner and achieved maximal responses at 40 ng/ml concentration of PDGF. The PDGF concentration required to achieve maximal monocyte aggregation was 2-fold (60 mg/ml) of that found for PMNs. A positive correlation (.gamma. = 0.93; P < 0.01) was observed between the increases of PDGF concentration and .beta.-glucuronidase release. Evidently, PDGF can induce the full sequence of cell activation events in human monocyte similar to human PMN.