Screening Peanut Germ Plasm Lines by Enzyme-Linked Immunosorbent Assay for Seed Transmission of Peanut Mottle Virus

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Abstract
Enzyme-linked immunosorbent assay (ELISA) was successfully employed for detecting peanut mottle virus (PMV) in peanut seed. Virus could be detected in extracts of infected cotyledons and embryos at dilutions of up to 1/3600, although sensitivity was reduced when infected and healthy seed were mixed. PMV was detected in the testa of about 30% of infected seed. Complete correlation was observed among ELISA, infectivity assays, and growing-out tests for all 4 peanut cultivars tested. PMV was seed-transmitted in all but two, EC 76446 (292) and NC AC 17133 (RF), of 283 peanut germ plasm lines screened.