Purification and properties of whale thyroid-stimulating hormone. III. Properties of isolated multiple components.

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Abstract
Properties of the 4 purified components of whale thyroid-stimulating hormone (TSH) were compared. The amino acid composition shows close similarity among these components. Their hexosamine and sialic acid contents are of the of the same magnitude, whereas the neutral sugar composition differs somewhat from each other. The MW of whale TSH determined by sedimentation equilibrium is 29,000, and no difference in MW and in Stokes radius as determined by gel filtration was detected among these 4 components. The amino acid and carbohydrate compositions of whale TSH resemble those of TSH from other species, especially those of non-primate mammalian TSH. Whale TSH contains, unlike bovine TSH but like human TSH, 1-2 residues of sialic acid as a constituent carbohydrate.