REMOVAL OF CONTAMINATING ERYTHROCYTES FROM HUMAN POLYMORPHONUCLEAR LEUKOCYTE PREPARATIONS
- 1 January 1983
- journal article
- research article
- Vol. 43 (7) , 621-627
Abstract
A simple discontinuous gradient of Percoll was used to remove the erythrocyte (RBC) contamination in human polymorphonuclear leukocyte (PMN) preparations avoiding the possible adverse effects of differential lysis. Mononuclear cells are also removed leaving a preparation with a white cell purity of more than 99% PMN contaminated by less than 2% RBC. The yield of PMN was 40% with respect to whole blood. The metabolic response of cells prepared in this way was found to be comparable with PMN isolated using the technique of Boyum. Small differences in the iodination response of the purified PMN were shown to be the result of the removal of RBC rather than an inherent effect of the Percoll isolation procedure.This publication has 9 references indexed in Scilit:
- RAPID SINGLE-STEP METHOD FOR PURIFICATION OF POLYMORPHONUCLEAR LEUKOCYTES FROM BLOOD OF PATIENTS WITH RHEUMATOID-ARTHRITIS1982
- AN IMPROVED METHOD FOR CONCURRENT ISOLATION OF HUMAN-GRANULOCYTES AND LYMPHOCYTES - ITS APPLICATION IN ISOELECTRIC-FOCUSING ELECTROPHORESIS1981
- Specific high affinity cell membrane receptors for biologically active phorbol and ingenol estersNature, 1980
- Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human blood by the hypaque-ficoll methodJournal of Immunological Methods, 1980
- Concurrent isolation of granulocytes and lymphocytes with unaltered permeability, energy state, and metabolic capacity in vitroBiochemical Medicine, 1980
- A rapid single centrifugation step method for the separation of erythrocytes, granulocytes and mononuclear cells on continous density gradients of percollJournal of Immunological Methods, 1980
- Separation of granulocytes from peripheral blood in a single step using discontinuous density gradients of Ficoll-Urografin. A comparative study with separation by dextranJournal of Immunological Methods, 1979
- A rapid one-step procedure for purification of mononuclear and polymorphonuclear leukocytes from human blood using a modification of the hypaque-ficoll techniqueJournal of Immunological Methods, 1978
- An improved method for isolation of granulocytes from peripheral bloodJournal of Immunological Methods, 1978