Specific binding of formylated initiator-tRNA to Escherichia coli RNA polymerase.
- 1 September 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (9) , 3064-3067
- https://doi.org/10.1073/pnas.73.9.3064
Abstract
E. coli fMet-tRNAfMEet and E. coli RNA plymerase (RNA nucleotidyltransferase; EC 2.7.7.6; nucleoside-triphosphate:RNA nucleotidyltransferase) form a 1:1 complex with an apparent association constant of 9.0 X 10(6)M-1 at 37 degrees. The affinity of polymerase to tRNA depends on the tRNA as well as the formyl methionine moiety. Core polymerase has a greatly reduced affinity for initiator tRNA. Optimal binding conditions are similar to those that are also optimal for binding initiator tRNA to ribosomes. Binding of initiator tRNA to polymerase stimulates the transcription of lambda plac DNA, as determined in a crude cell-free system for beta-galactosidase (EC 3.2.1.23; beta-D-galactoside galactohydrolase) synthesis as well as in a highly purified transcription system.This publication has 11 references indexed in Scilit:
- The codon binding site of the Escherichia coli ribosome as studied with a chemically reactive A-U-G analogJournal of Molecular Biology, 1975
- Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase).Proceedings of the National Academy of Sciences, 1975
- A link between streptomycin and rifampicin mutation.Proceedings of the National Academy of Sciences, 1975
- Recognition of normal and modified tRNA by streptomycin sensitive and resistant ribosomes of Escherichia coliMolecular Genetics and Genomics, 1974
- Does formylation of initiator tRNA act as a regulatory signal in E. coli?FEBS Letters, 1973
- Control of Ribosomal RNA Synthesis in vitroNature, 1973
- MSI and MSII made on Ribosome in Idling Step of Protein SynthesisNature, 1972
- Affinity Chromatography of DNA‐Binding Enzymes on Single‐Stranded DNA‐Agarose ColumnsEuropean Journal of Biochemistry, 1972
- A New Method for the Large Scale Purification of Escherichia coli Deoxyribonucleic Acid-dependent Ribonucleic Acid PolymeraseJournal of Biological Chemistry, 1969
- Isolation of Pure lac Operon DNA*Nature, 1969