ON THE IDENTIFICATION OF GLYCOGEN IN ELECTRON MICROGRAPHS

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Abstract
Gylcogen is identified in electron micrographs of osmium fixed mouse liver by an application of a freezing-thawing technique (Baker R.F, J. of Ultrastruc. Res. 7, 173, 1962). Cryostat sections 10 [mu] thick were cut from quick frozen mouse liver at -18[degree] C and one group of sections was thawed in distilled water followed by 15 min. OSO4 fixation with the other group thawed in 1% [alpha]-amylase in distilled water. Two min. incubation in [alpha]-amylase of the latter group was followed by 15 min. OSO4 fixation. Ultra-thin sections were cut from the Epon embedded tissue. Electron micrographs of the control sections showed typical intra-cytoplasmic groups of particles, which were uniformly missing in the enzyme treated tissue. The conclusion drawn was that the groups of particles represented glycogen in an extractable form.

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