Interaction Between Endothelin-Induced Na+ and Ca2+ Kinetics in Cultured Rat Vascular Smooth Muscle Cells
- 1 January 1991
- journal article
- opening lecture
- Published by Wolters Kluwer Health in Journal of Cardiovascular Pharmacology
- Vol. 17, S124-126
- https://doi.org/10.1097/00005344-199100177-00033
Abstract
Summary The present study was undertaken to examine the interaction between endothelin-1 (ET-1)-induced Ca2+ and Na+ kinetics in cultured rat vascular smooth muscle cells (VSMCs) by measuring cytosolic free Ca2+ ([Ca2+]i) and intracellular Na+ ([Na+]i) using intracellular fluorescent dyes (fura-2 and SBFI, respectively). ET-1 increased both [Ca2+]i (basal. 58.9 n M; 10-9M, 119.8 n M; 10-8M, 265.0 n M; 10-7M, 419.0 n M) and [Na+]i (basal, 13.4 m M; 10-9M, 17.1 m M; 10-8M, 21.6 m M; 10-7M, 25.2 m M) in a dose-dependent manner in cultured rat VSMCs. The Na+ -free solution enhanced the 10-7M ET-1-mobilized [Ca2+]i (419 vs. 1,192 n M) but reduced the 10-7M ET-1-induced increase in [Na+]i (25.2 vs. 6.1 m M). The Ca2+ -free solution decreased the peak levels of 107M ET-1-induced mobilization of [Ca2+]i (411 vs. 350 n M) and the sustained increase in [Ca2+]i and totally diminished the 10-7M ET-1-induced increase in [Na+]i (25.2 vs. 12.8 m M). These results therefore indicate that ET-1 increases [Na+]i in association with an increase in cellular Na+ and Ca2+ uptake in VSMCs.Keywords
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