A COMPARISON OF SEVERAL EXTRACTION PROCEDURES FOR RABBIT ACROSOMAL ENZYMES

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Abstract
A variety of enzymes, lipids and polysaccharides have been described as `acrosomal' and implicated in the fertilization process (Hathaway & Hartree, 1963; Srivastava, Adams & Hartree, 1965; Hartree & Srivastava, 1965; Stambaugh & Buckley, 1968, 1969; Allison & Hartree, 1970; Zaneveld, Srivastava & Williams, 1969; Zaneveld, Polakoski & Williams, 1972). Several extraction procedures have been used specifically for removing the acrosomal contents, and it seemed appropriate to compare and reinvestigate their specificity. It was hoped that this comparison might provide some insight into the specificity of each procedure, and the need for additional criteria to label an extract or enzyme as `acrosomal'. The first method examined involved a direct extraction of washed spermatozoa with detergents. By this procedure, epididymal spermatozoa were washed three times with isotonic saline,

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