AUTORADIOGRAPHIC DISTRIBUTION STUDIES WITH 14C-CORTISONE AND 14C-CORTISOL

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Abstract
The distribution of C14-labelled cortisone and cortisol was investigated by wholebody autoradiog-raphy in mice. No clear difference in distribution pattern was found between the 2 substances. Both were rapidly distributed in all tissues. Apart from organs such as liver, kidney and intestines, selective accumulation was observed in bronchial mucosa, ductus epididymis, vas deferens, interstitial cells of testicle and ovary, and epithelium of the yolk sac. Fairly high concentrations were found in brown fat and in pituitary gland. Both radioactive substances penetrated the placental barrier, but uptake was lower in foetuses than in maternal tissues. The adrenal cortex was the only organ in fetuses to show accumulation. Very little activity was seen in adrenal cortex of the dams. Radioactivity in joints (cartilage and synovial fluid) was low. The sclera, iris and superficial cell layers of the lens showed accumulation of activity. The radioactive substances were seen in choroid plexus and in cerebro-spinal fluid. They were evenly but very scantily distributed in brain tissue. The formation and distribution of metabolites are discussed.