Exogenous expression of claudin‐5 induces barrier properties in cultured rat brain capillary endothelial cells
- 22 September 2006
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 210 (1) , 81-86
- https://doi.org/10.1002/jcp.20823
Abstract
Claudins are thought to be major components of tight junctions (TJs), and claudin‐5 and ‐12 are localized at TJs of the blood‐brain barrier (BBB). Claudin‐5‐deficient mice exhibit size‐selective (14C]inulin at a TR‐BBB/CLD5 cell monolayer was significantly smaller (P < 0.01) than that for the parental TR‐BBB cells, and the values of the permeability coefficient (Pe) were 1.14 × 10−3 and 11.6 × 10−3 cm/min, respectively. These results indicate that claudin‐5, but not claudin‐12, is predominantly expressed in brain capillaries, and plays a key role in the appearance of barrier properties of brain capillary endothelial cells. J. Cell. Physiol. 210: 81–86, 2007.Keywords
This publication has 20 references indexed in Scilit:
- Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cellsThe Journal of cell biology, 2005
- Selective gene silencing of rat ATP-binding cassette G2 transporter in an in vitro blood-brain barrier model by short interfering RNAJournal of Neurochemistry, 2005
- A pericyte‐derived angiopoietin‐1 multimeric complex induces occludin gene expression in brain capillary endothelial cells through Tie‐2 activation in vitroJournal of Neurochemistry, 2004
- Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contactsCell and tissue research, 2003
- mRNA Expression and Transport Characterization of Conditionally Immortalized Rat Brain Capillary Endothelial Cell Lines; a Newin vitroBBB Model for Drug TargetingJournal of Drug Targeting, 2000
- A Single Gene Product, Claudin-1 or -2, Reconstitutes Tight Junction Strands and Recruits Occludin in FibroblastsThe Journal of cell biology, 1998
- Claudin-1 and -2: Novel Integral Membrane Proteins Localizing at Tight Junctions with No Sequence Similarity to OccludinThe Journal of cell biology, 1998
- Occludin: a novel integral membrane protein localizing at tight junctions.The Journal of cell biology, 1993
- A One‐Step Procedure for Isolation of Poly(A)+ mRNA from Isolated Brain Capillaries and Endothelial Cells in CultureJournal of Neurochemistry, 1991
- An Easier, Reproducible, and Mass‐Production Method to Study the Blood–Brain Barrier In VitroJournal of Neurochemistry, 1990