Purification and some properties of starch branching enzyme (Q‐enzyme) from tuberous root of sweet potato
- 1 August 1994
- journal article
- Published by Wiley in Physiologia Plantarum
- Vol. 91 (4) , 763-769
- https://doi.org/10.1111/j.1399-3054.1994.tb03017.x
Abstract
The pattern of isoforms of starch branching enzyme II or Q‐enzyme II in the tuberous root of sweet potato was distinct from those of other organs; altogether 7 isoforms of QEII were contained in the sweet potato plant. The QEIIf isoform, one of the two major QEII isoforms in the tuberous root, was purified to homogeneity by using a variety of HPLC columns. The purified QEIIf was a monomeric protein with a molecular mass of about 85 kDa. Western blot analysis showed that the polyclonal antibodies raised against the purified QEIIf was significantly reactive to the rice endosperm QEI, but not to the rice endosperm QEIIa. Furthermore, the sweet potato QEIIf reacted with the antiserum raised against the rice endosperm QEI, but not with that against the rice endosperm QEIIa. The results suggest that the sweet potato QEIIf is more similar to the rice endosperm QEI than to the rice endosperm QEIIa.Keywords
This publication has 9 references indexed in Scilit:
- Differentiation of the Properties of the Branching Isozymes from Maize (Zea mays)Plant Physiology, 1993
- Purification of two forms of starch branching enzyme (Q‐enzyme) from developing rice endospermPhysiologia Plantarum, 1992
- Relationship between Starch Content and Activity of Starch Synthase and ADP-glucose Pyrophosphorylase in Tuberous Root of Sweet Potato.Japanese Journal of Crop Science, 1992
- Major differences in isoforms of starch-branching enzyme between developing embryos of round- and wrinkled-seeded peas (Pisum sativum L.)Planta, 1988
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Multiple forms of (1 → 4)-α-d-glucan, (1 → 4)-α-d-glucan-6- glycosyl transferase from developing zea mays L. KernelsCarbohydrate Research, 1978
- Interaction of spinach leaf adenosine diphosphate glucose α-1,4-glucan α-4-glucosyl transferase and α-1,4-glucan, α-1,4-glucan-6-glycosyl transferase in synthesis of branched α-glucanArchives of Biochemistry and Biophysics, 1974
- Isolation and characterization of the potato α-1,4-glucan-α-1,4-glucan 6-glucosyltransferaseBiochemistry, 1968
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964