Isolation and Characterization of a SCT1 Gene Which Can Suppress a Choline-Transport Mutant of Saccharomyces cerevisiae
- 1 February 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 117 (2) , 447-451
- https://doi.org/10.1093/jb/117.2.447
Abstract
The yeast Saccharomyces cerevisiae possesses a choline-transport system encoded by the CTR1 gene. We isolated a yeast gene, SCT1, that can suppress a CTR1 defect when introduced as a multicopy. The SCT1 coding frame is capable of encoding 759 residues with a calculated molecular weight of 85,734. On Northern blot analysis, an RNA species that hybridized with the coding region was detected in the total RNA of the wild-type yeast. The transcription of SCT1 is constitutive. The primary translation product contains three membrane-spanning domains, a PEST-like sequence, and a glutamic acid-rich sequence at the C terminal end. Gene disruption experiments showed that SCT1 is not an essential gene under the standard culture conditions. SCT1 did not suppress a null mutant of ctrl, indicating that a mutant form of choline transporter is necessary for the suppression caused by SCT1.Keywords
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