Renaturation, activation, and practical use of recombinant duplex-specific nuclease from Kamchatka crab

1 May 2006

journal article
Published by Pleiades Publishing Ltd in Biochemistry (Moscow)

Vol. 71 (5) , 513-519
https://doi.org/10.1134/s0006297906050075

Abstract

We overexpressed duplex-specific nuclease (DSN) from Kamchatka crab in Escherichia coli cells and developed procedures for purification, renaturation, and activation of this protein. We demonstrated identity of the properties of the native and recombinant DSN. We also successfully applied the recombinant DSN for full-length cDNA library normalization.

Keywords

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