HYDROLYSIS OF PHENYL BETA-MALTOSIDE CATALYZED BY SACCHARIFYING ALPHA-AMYLASE FROM BACILLUS-SUBTILIS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 81  (5) , 1187-1192
Abstract
The hydrolytic reaction of phenyl .beta.-maltoside catalyzed by saccharifying .alpha.-amylase [EC 3.2.1.1] of B. subtilis was studied at 25.degree. C and pH 5.4, by measuring the total reducing power and the amount of phenol liberated, and by TLC. The enzyme hydrolyzed phenyl .beta.-maltoside at the glucosidic linkage between the 2 glucose residues to form D-glucose and phenyl .beta.-D-glucoside. Besides these products, maltose, maltotriose and phenyl .beta.-maltotrioside were observed as reaction products. The identification of phenyl .beta.-maltotrioside is described in detail. The formation of these products was attributed to the transglycosylation reaction of the enzyme. The time course of reaction as followed by reducing power measurement showed an induction period of several minutes.