In vitro Synthesis of Mycorrhizae between Presumed Specific and Nonspecific Pinus + Suillus Combinations

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Abstract
Mycorrhizal synthesis was successful between P. resinosa and S. americanus, S. brevipes (2 isolates), S. luteus and S. neoalbidipes, and between P. strobus and S. americanus, S. brevipes (2 isolates), S. granulatus, S. pictus and S. punctipes. Mycorrhizae formed by all combinations were similar to that all had multiple dichotomous branches, hyphae and hypal strands with microscopic, pigmented, surface incrustations surrounding the mycorrhizae and roots, and in the mantle organization. Mycorrhizae differed in the macroscopic color of the mantle and surrounding hyphae and hyphal strands, the amount of hypal surface incrustation present, the organization of individual hyphae in the mantle layers, the width of the mantle, the presence of clamp connections, and the amount of radial elongation of cortical cells. Data on additional diagnostic characters must be collected and evaluated for value in the identification of mycobionts of field-collected mycorrhizae.