Identification of novel heat shock factor‐dependent genes and biochemical pathways in Arabidopsis thaliana
Open Access
- 17 November 2004
- journal article
- Published by Wiley in The Plant Journal
- Vol. 41 (1) , 1-14
- https://doi.org/10.1111/j.1365-313x.2004.02272.x
Abstract
Summary: In order to assess specific functional roles of plant heat shock transcription factors (HSF) we conducted a transcriptome analysis of Arabidopsis thaliana hsfA1a/hsfA1b double knock out mutants and wild‐type plants. We used Affymetrix ATH1 microarrays (representing more than 24 000 genes) and conducted hybridizations for heat‐treated or non‐heat‐treated leaf material of the respective lines. Heat stress had a severe impact on the transcriptome of mutant and wild‐type plants. Approximately 11% of all monitored genes of the wild type showed a significant effect upon heat stress treatment. The difference in heat stress‐induced gene expression between mutant and wild type revealed a number of HsfA1a/1b‐regulated genes. Besides several heat shock protein and other stress‐related genes, we found HSFA‐1a/1b‐regulated genes for other functions including protein biosynthesis and processing, signalling, metabolism and transport. By screening the profiling data for genes in biochemical pathways in which known HSF targets were involved, we discovered that at each step in the pathway leading to osmolytes, the expression of genes is regulated by heat stress and in several cases by HSF. Our results document that in the immediate early phase of the heat shock response HSF‐dependent gene expression is not limited to known stress genes, which are involved in protection from proteotoxic effects. HsfA1a and HsfA1b‐regulated gene expression also affects other pathways and mechanisms dealing with a broader range of physiological adaptations to stress.Keywords
This publication has 47 references indexed in Scilit:
- Tomato Heat Stress Transcription Factor HsfB1 Represents a Novel Type of General Transcription Coactivator with a Histone-Like Motif Interacting with the Plant CREB Binding Protein Ortholog HAC1[W]Plant Cell, 2004
- The Role of Heat Shock Transcription Factor 1 in the Genome-wide Regulation of the Mammalian Heat Shock ResponseMolecular Biology of the Cell, 2004
- Dissection of floral induction pathways using global expression analysisDevelopment, 2003
- Two different heat shock transcription factors regulate immediate early expression of stress genes in ArabidopsisMolecular Genetics and Genomics, 2003
- Exploration, normalization, and summaries of high density oligonucleotide array probe level dataBiostatistics, 2003
- The Effects of Polyethylene Glycol on Gene Expression of Developing White Spruce Somatic EmbryosPlant Physiology, 2003
- The Role of AHA Motifs in the Activator Function of Tomato Heat Stress Transcription Factors HsfA1 and HsfA2Plant Cell, 2000
- The promoter of a H2O2‐inducible, Arabidopsis glutathione S‐transferase gene contains closely linked OBF‐ and OBP1‐binding sitesThe Plant Journal, 1996
- Derepression of the activity of genetically engineered heat shock factor causes constitutive synthesis of heat shock proteins and increased thermotolerance in transgenic ArabidopsisThe Plant Journal, 1995
- Regulation of the transcription of heat shock genes in nuclei from soybean (Glycine max) seedlingsPlant, Cell & Environment, 1987