Identification of mycobacteria by high-performance liquid chromatography
- 1 November 1991
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 29 (11) , 2468-2472
- https://doi.org/10.1128/jcm.29.11.2468-2472.1991
Abstract
Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a differentiation scheme that was 98% accurate for the species examined. A rapid, cost-effective HPLC method which offers an alternative approach to the identification of mycobacteria is described.Keywords
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