Histochemical Distribution of Alkaline Phosphatase in Dog Liver After Experimental Biliary Obstruction

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Abstract
Bile capillaries showed distinct phosphatase activity in the normal dog liver. Gomori''s method (Proc. Soc. Exp. Biol. and Med. 1939 42, 23) as modified by Kabat and Furth (Am. J. Path., 1941, 17, 303) was used for the histochemical demonstration of alkaline phosphatase activity. After ligation of the common bile duct there was not only marked dilatation of the bile capillaries but also apposition of phosphatase around them which increased with the duration of the expt. The changes in the distribution of alkaline phosphatase in histochemical prepns. favored the assumption that the increase of serum phosphatase in liver damage was due to the inability of the liver cells to excrete the enzyme rather than to increased production in the liver itself.