Immunochemical and Electrophoretic Properties of Equine Luteinizing Hormone1

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Abstract
Antiserum to equine LH was produced in 10 rabbits to determine the extent to which it would cross-react with LH from other species and with other pituitary hormones. Antiserum, after absorption with equine blood serum, resulted in a single precipitation when diffused against as little as 0.8 µ. equine LH in agar gel. This precipitation line for equine LH was confluent with a similar line for PMS and with similar lines for saline extracts of bovine, ovine, and rat pituitaries, respectively. The antiserum failed to react with other hormones purified from the anterior pituitary, with HCG or with saline extract of chicken pituitaries. Immunoelectrophoresis and complement fixation tests generally substantiated the conclusion from agar gel double diffusion studies that antiserum to equine LH reacted with each substance that was expected to contain appreciable quantities of LH. Ovarian ascorbic acid depletion asays of purified equine, bovine and ovine LH as well as saline extracts of bovine, ovine, and rat pituitaries and mixtures of each of these sources of LH with antiserum to equine LH revealed that the antiserum neutralized the physiological activity of each of these sources of LH. Copyright © 1965. American Society of Animal Science . Copyright 1965 by American Society of Animal Science