Efficient generation of antibodies to oncoproteins by using synthetic peptide antigens.
- 1 May 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (10) , 3400-3404
- https://doi.org/10.1073/pnas.82.10.3400
Abstract
To examine the efficiency of generating protein-reactive antipeptide antibodies, 35 peptides encoded by retroviral or cellular oncogenes were used to immunize rabbits. Peptides (32) elicited antipeptide antibodies, of which 56% reacted with their respective oncoproteins. The length of the immunizing peptide was an important factor in generating antibodies reactive with native protein. Similar peptides differing in a single or a few amino acids could elicit antisera of markedly different reactivities.This publication has 27 references indexed in Scilit:
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