Stepwise ring closure in penicillin biosynthesis. Intitial β-lactam formation
- 1 January 1984
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Chemical Communications
- No. 15,p. 984-986
- https://doi.org/10.1039/c39840000984
Abstract
Incubation of a 1 : 1 mixture of δ-(L-α-aminoadipoyl)-L-cysteinyl-D-valine (L,L,D-ACV) and L,L,D-A[3,3-2H2]CV with a cell-free extract of isopenicillin N synthetase from Cephalosporium acremonium results in preferential conversion of the fully protiated substrate into isopenicillin N; alternatively a similar experiment with a 1 : 1 mixture of L,L,D-ACV and L,L,D-AC[3-2H]V gave isopenicillin N without isotopic discrimination between the two substrates.Keywords
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