Cellular localization of the Ro/SS-A antigen
- 1 March 1986
- journal article
- conference paper
- Published by Springer Nature in Clinical Rheumatology
- Vol. 5 (1) , 33-38
- https://doi.org/10.1007/bf02030965
Abstract
The cellular localization of the Ro/SS-A antigen was defined using indirect immunofluorescence and eight monospecific anti-Ro/SS-A antisera which were identified by immunoblotting. Several mammalian tissues were used as substrates. The Ro/SS-A antigen was located mainly in the nucleus of dog liver and Hep-2-cells, and anti- Ro sera had produced a speckled staining pattern. Cytoplasmic fluorescence appeared only in one serum. IgG was the predominant immunoglobulin with anti-Ro/SS-A activity;five sera had complement fixing activity. The sera absorption studies with partially purified Ro/SS-A antigen and the negativation of the ANA tests had confirmed the specificity of our findings.This publication has 20 references indexed in Scilit:
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