A Missense Mutation Accounts for the Defect in the Glycerol-3-Phosphate Acyltransferase Expressed in the plsB26 Mutant
- 15 March 1999
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 181 (6) , 1944-1946
- https://doi.org/10.1128/jb.181.6.1944-1946.1999
Abstract
The sn -glycerol-3-phosphate acyltransferase ( plsB ) catalyzes the first step in membrane phospholipid formation. A conditional Escherichia coli mutant ( plsB26 ) has a single missense mutation (G1045A) predicting the expression of an acyltransferase with an Ala349Thr substitution. The PlsB26 protein had a significantly reduced glycerol-3-phosphate acyltransferase specific activity coupled with an elevated K m for glycerol-3-phosphate.Keywords
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