Studies on the Immunoglobulins Which Stimulate the Ingestion of Glutaraldehyde-Treated Red Cells Attached to Macrophages

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Abstract
Summary: Fractions obtained from anti-horse red cell rabbit sera were examined as to their ability to stimulate the ingestion of glutaraldehydetreated horse red cells (GRC) attached to macrophages. Activity of both primary (10-day) and secondary sera was restricted to the light (7 S) fractions obtained by means of sucrose density gradient ultracentrifugation. Preparations rich in IgM were obtained from 7-day primary immune response serum by means of salt precipitation, gel filtration and gradient density centrifugation. These preparations were found to be inactive in the ingestion system, in contrast to chromatographically purified IgG obtained from secondary response serum. The ingestion of GRC treated with IgM or with 7 S globulins was markedly stimulated when carried out in the presence of anti-rabbit globulin goat serum. Treatment of IgG with pepsin markedly reduced the ingestion stimulating activity.